{"id":3118,"date":"2026-05-21T12:00:00","date_gmt":"2026-05-21T12:00:00","guid":{"rendered":"https:\/\/cicds.umfcd.ro\/?post_type=lucrari_cercetari&p=3118"},"modified":"2026-08-05T15:48:45","modified_gmt":"2026-08-05T15:48:45","slug":"biocompatibility-and-cell-death-mechanisms-induced-by-pmma-based-dental-materials-in-gingival-fibroblasts-and-oecm-1-tumor-cells","status":"publish","type":"lucrari_cercetari","link":"https:\/\/cicds.umfcd.ro\/en\/lucrari_cercetari\/biocompatibility-and-cell-death-mechanisms-induced-by-pmma-based-dental-materials-in-gingival-fibroblasts-and-oecm-1-tumor-cells\/","title":{"rendered":"Biocompatibility and Cell Death Mechanisms Induced by PMMA-Based Dental Materials in Gingival Fibroblasts and OECM-1 Tumor Cells"},"content":{"rendered":"
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Biocompatibility and Cell Death Mechanisms Induced by PMMA-Based Dental Materials in Gingival Fibroblasts and OECM-1 Tumor Cells<\/h1>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Rus, F.; Radulescu, R.; Popa, A.; Voicu-Balasea, B.; Musteanu, M.; Izet, M.; Muscurel, C.; Ciocan, L.T.; Bancu, S.-A.; Cernega, A.; Ripszky, A.; Pituru, S.-M.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Dentistry Journal 2026, 14, 315.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\t\tAbstract\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Background\/Objectives:<\/b> The present study aims to test three different types of PMMA (Fotodent Guide\u20143D printed (M1), Aidite Temp\u2014milled (M2), Duracryl\u2014self-polymerized (M3) on HFIB-G and on OECM-1. Methods:<\/b> The two cell types (HFIB-G and OECM-1) were kept in contact with the materials, Fotodent Guide, Aidite Temp, and Duracryl (n<\/span> = 6), for 24 and 48 h, and subsequently subjected to the following tests: MTT, LDH, NO (according to ISO 10993-5:2009), and immunofluorescent detection of proteins associated with autophagy and apoptosis (mitochondria and caspases 3\/7; detection of autophagosomes). Statistical interpretation was made using t-test and ANOVA (* p<\/span> < 0.05; ** p<\/span> < 0.01; *** p<\/span> < 0.001). Results:<\/b> The MTT assay revealed a reduction in cell viability for all tested materials on gingival fibroblasts compared to control cells, with the most pronounced decrease observed for the 3D-printed material (M1 viability 66.77% for 24 and 52.45% 48 h\u2014p<\/span> < 0.001), while the self-polymerizing resin (M3 viability 85.92% for 24 h and 85.63% for 48 h) showed the highest level of cellular tolerance (p<\/span> < 0.001 at 24 h and p<\/span> < 0.01 at 48 h). Regarding OECM-1 cells, all materials reduced cell viability, particularly M3 after 48 h of incubation (viability 61.79%\u2014p<\/span> < 0.001). LDH levels generally indicated low membrane damage for all materials. Statistically significant increases in NO levels were recorded for both cell types, suggesting a mild proinflammatory response, especially for M2 OECM-1 48 h\u2014p<\/span> < 0.05 and M3 (HFIB-G 48 h\u2014p<\/span> < 0.05, OECM-1 48 h p<\/span> < 0.05). For both 24 and 48 h, fluorescence analysis demonstrated a significant increase in mitochondrial activity in gingival fibroblasts (p<\/span> < 0.001), whereas tumor cells exhibited a significantly decreased mitochondrial activity (p<\/span> < 0.001), particularly for the 3D-printed material M1 (p<\/span> < 0.001). Caspase-3\/7 expression increased in gingival fibroblasts incubated with materials for 24 and 48 h (p<\/span> < 0.001), while tumor cells showed reduced caspase activity both after 24 and 48 h (p<\/span> < 0.001). Autophagosome formation decreased initially in fibroblasts at 24 h (p<\/span> < 0.001) but increased significantly after 48 h (p<\/span> < 0.001), while tumor cells generally showed enhanced autophagic activity under most experimental conditions (p<\/span> < 0.001). Conclusions:<\/b> Our results suggest that all three PMMA-based materials exhibit acceptable biocompatibility profiles, of more than 70%, according to ISO 10993-5:2009, although cellular responses vary depending on the manufacturing technique and the cellular model used. In our study conditions, self-polymerized resin (M3) was the most compatible with gingival fibroblasts, while the 3D-printed and CAD\/CAM milled materials (M1 and M2) had a more pronounced impact on cells\u2019 viability and metabolic activity.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Keywords: <\/strong><\/p>

3D printing<\/a>; <\/a>PMMA<\/a>; <\/a>human oral squamous carcinoma cell line<\/a>; <\/a>biocompatibility<\/a>; <\/a>autophagy<\/a>; <\/a>apoptosis<\/a>; <\/a>human gingival fibroblasts<\/a>; <\/a>provisional prosthetic restauration<\/a><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t<\/path><\/svg>\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\tRead the paper online<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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